BIOLSERV-L Archives

April 2014

BIOLSERV-L@LISTSERV.GMU.EDU

Options: Use Monospaced Font
Show HTML Part by Default
Condense Mail Headers

Message: [<< First] [< Prev] [Next >] [Last >>]
Topic: [<< First] [< Prev] [Next >] [Last >>]
Author: [<< First] [< Prev] [Next >] [Last >>]

Print Reply
Content-Type:
multipart/alternative; boundary="_000_6b8f68659ba0491b9eff2b584c7094afCO1PR05MB474namprd05pro_"
Subject:
From:
"Diane St. Germain" <[log in to unmask]>
Date:
Thu, 10 Apr 2014 20:07:50 +0000
MIME-Version:
1.0
Comments:
To: BIOSCIENCES <[log in to unmask]>, "[log in to unmask]" <[log in to unmask]>, SSB Faculty <[log in to unmask]>, Claudius Mueller <[log in to unmask]> cc: Peggy A Hackett <[log in to unmask]>, Jacqueline M Houle <[log in to unmask]>, Andrea George <[log in to unmask]>, Jennifer Bazaz <[log in to unmask]>
Reply-To:
"Diane St. Germain" <[log in to unmask]>
Parts/Attachments:
text/plain (2753 bytes) , text/html (7 kB)

Thesis Defense Announcement
To:  The George Mason University Community

Candidate: Khoa Tran

Program: M.S. in Biology



Date:   Thursday April 24, 2014

Time:   1:00 p.m.

Place:  George Mason University
             Prince William Campus<http://www.gmu.edu/resources/welcome/Directions-to-GMU.html>

             Bull Run Hall, Room 258



Title: "Simultaneous Application of Chromosomal Microarray Analysis and Polymerase Chain Reaction Genetic Disease Detection"
Thesis Director: Dr. Lance A. Liotta
Thesis Committee:  Dr. Claudius Mueller, Dr. Brian D. Mariani
A copy of the thesis will be available in the Mercer Library.  All are invited to attend the defense.
ABSTRACT
This thesis demonstrates the successful integration and application of chromosomal microarray analysis (CMA) and mutation-specific polymerase chain reaction (PCR) within 24 hours for the detection of unaffected euploid embryos from couples at risk for genetic disease undergoing in vitro fertilization treatment. Currently, array comparative genomic hybridization (aCGH) for detecting aneuploidy and PCR, and/or sequencing, for mutation status detection are performed at two different time points, thus, not appropriate for delivering results within a time frame suitable for a fresh embryo transfer. The techniques presented here have been optimized for analysis and reporting within a 24-hr time frame, allowing clinicians to offer their patients the option of fresh embryo transfer.
    Whole genome amplification (WGA) of trophectoderm embryo biopsy was performed and aliquots of amplified DNA products were divided for CMA and for laboratory-developed fluorescent PCR coupled with capillary electrophoresis for genetic disease diagnosis. The combined technique requires no modification of the CMA protocol and no pre-amplification steps were required prior to WGA. The WGA-amplified DNA products were subjected to single round PCR testing for mutational and linked marker detection, except for mutations involving triplet repeats where nested PCR was necessary.
    Although current techniques are available for detecting aneuploidy and genetic mutation status, such as CGH or SNP arrays for aneuploidy and PCR or sequencing for gene mutation, these methods cannot be combined for analysis within a 24-hour time frame. The successful combination of these important techniques provides a comprehensive diagnostic approach that can be offered to couples at risk for single-gene disorders wishing to receive a fresh embryo transfer. This new approach allows laboratories currently equipped to perform aCGH and PCR to utilize their existing setup for a novel comprehensive diagnostic protocol without additional equipment acquisition.
 ###



ATOM RSS1 RSS2