April 2014


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"Diane St. Germain" <[log in to unmask]>
Wed, 2 Apr 2014 15:47:10 +0000
To: BIOSCIENCES <[log in to unmask]>, "[log in to unmask]" <[log in to unmask]>, "A. Alonso Aguirre" <[log in to unmask]>, Pat Gillevet <[log in to unmask]> cc: Peggy A Hackett <[log in to unmask]>, Jacqueline M Houle <[log in to unmask]>, Andrea George <[log in to unmask]>, Jennifer Bazaz <[log in to unmask]>
"Diane St. Germain" <[log in to unmask]>
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Thesis Defense Announcement
To:  The George Mason University Community

Candidate: Jennifer Ravinskas

Program: Master of Science in Biology

Date:   Wednesday April 15, 2014

Time:   10:00 a.m.

Place:  George Mason University
             Prince William Campus<>

             Occoquan Bldg., Room 110-H

Title: "The Genetic Variation of Peregrine Falcons (Falco peregrinus) in the Chesapeake Bay"
Thesis Director: Dr. Alonso Aguirre
Thesis Committee:  Dr. Ancha Baranova, Dr. Patrick Gillevet
A copy of the thesis will be available in the Mercer Library.  All are invited to attend the defense.
For the last century, the local population of Chesapeake Bay peregrine falcons (Falco peregrinus) has been fluctuating in its abundance, making genetic diversity difficult to assess (Johnson et al., 2010). Recently, the well-being of falcons in Coastal Virginia has improved, and their populations have been stabilizing in numbers. The extent of the genetic variation in the Chesapeake Bay population of falcons is unknown, yet the possibility of a genetic bottleneck does exist. To assess levels of allelic heterozygosity in this population, genetic analyses were performed on blood and feather samples taken from peregrine falcons breeding in the Bay during the years 2007 and 2013. DNA was extracted from these samples then fingerprinted at microsatellite loci with peregrine specific labeled primers (Nesje 2000b). Fingerprint results were analyzed using the GenAlEx 6.5 software, then expected observed alleles (AO), heterozygosity (HE), observed heterozygosity (HO), and fixation index (FST) were assessed.  At each locus studied, there were similarities in HO between the contemporary populations in other global reintroduction programs in southern Scandinavia and southern Norway and the contemporary population of falcons in the Chesapeake Bay. In locus NVH fp54,  observed heterozygosity in the Chesapeake Bay population was dramatically lower than that in the European populations. In Chesapeake Bay population, FST values were close to the zero at each locus, suggesting complete panmixis of this population. The cluster analyses performed with the STRUCTURE 2.3.4 software confirmed that the contemporary populations of peregrine falcons in the Chesapeake Bay are effectively one large population. Comparisons were made between the historic population of peregrine falcons endemic to the east cast (the Appalachian mountains) and the contemporary population of birds reintroduced to and currently living in the Chesapeake Bay. These comparisons showed no significant difference when levels of observed heterozygosity, expected heterozygosity, and number of alleles were assessed. This suggests that the reintroduced population of peregrines is no more able to cope with anthropogenic or environmental disturbances than their historic counterparts. Known susceptibility of peregrine falcons to environmental contamination as well as relatively low heterozygosity levels in the contemporary Chesapeake Bay population of peregrine falcons suggest that continued monitoring and further conservation efforts of this population are warranted.