> Reminder: Thesis Defense
> To: the George Mason Community
>
> *Stephanie L. Coon
> Master of Science in Biology
> Molecular Biology concentration
> *
> Date: Thursday November 18, 2010
> Time: 4:00 - 5:30 P.M.
> Place: Bull Run Hall, Room 249
> Prince William campus <http://www.gmu.edu/resources/visitors/findex.html>
> <http://www.gmu.edu/resources/visitors/findex.html>
> Thesis Chair: Dr. Ancha Baranova, MMB Dept.
>
> *Title:
> * "A SEARCH FOR /KCNRG/ MUTATIONS IN MULTIPLE MYELOMA CELL LINES"_//_
> *Abstract:
> *
>
> Deletions and or rearrangements on chromosome 13q14.3 are observed in 
> more than half of multiple myeloma (MM) and chronic lymphocytic 
> leukemia (CLL) cases and are also frequently seen in other 
> hematopoietic malignancies. The minimal common deleted region (CDR) in 
> MM cells contains candidate tumor suppressor gene /KCNRG/ (potassium 
> channel regulating gene), the product of which suppresses assembly of 
> the Kv channels and Kv currents. /KCNRG/ exerts growth suppressive and 
> pro-apoptotic effects in HL-60, LNCaP and RPMI-8226 cells. In this 
> study, the /KCNRG/ gene was sequenced in three multiple myeloma cell 
> lines, NCI-H929, RPMI-8226 and U266B2. It was found that the RPMI-8226 
> cell line contains a delT mutation in the core promoter initiator 
> element. Deletion of T decreases matrix similarity of this DNA element 
> from 0.945 to 0.941, and, therefore, might negatively influence 
> expression of KCNRG in RPMI-8226 cells. This suggests that KCNRG 
> expression may be negatively influenced in this model line. The 
> haploinsufficiency of KCNRG might be relevant to the progression of 
> CLL and MM at least in a subset of patients.
>
>
> ###
> *
> *