> Reminder: Thesis Defense > To: the George Mason Community > > *Stephanie L. Coon > Master of Science in Biology > Molecular Biology concentration > * > Date: Thursday November 18, 2010 > Time: 4:00 - 5:30 P.M. > Place: Bull Run Hall, Room 249 > Prince William campus <http://www.gmu.edu/resources/visitors/findex.html> > <http://www.gmu.edu/resources/visitors/findex.html> > Thesis Chair: Dr. Ancha Baranova, MMB Dept. > > *Title: > * "A SEARCH FOR /KCNRG/ MUTATIONS IN MULTIPLE MYELOMA CELL LINES"_//_ > *Abstract: > * > > Deletions and or rearrangements on chromosome 13q14.3 are observed in > more than half of multiple myeloma (MM) and chronic lymphocytic > leukemia (CLL) cases and are also frequently seen in other > hematopoietic malignancies. The minimal common deleted region (CDR) in > MM cells contains candidate tumor suppressor gene /KCNRG/ (potassium > channel regulating gene), the product of which suppresses assembly of > the Kv channels and Kv currents. /KCNRG/ exerts growth suppressive and > pro-apoptotic effects in HL-60, LNCaP and RPMI-8226 cells. In this > study, the /KCNRG/ gene was sequenced in three multiple myeloma cell > lines, NCI-H929, RPMI-8226 and U266B2. It was found that the RPMI-8226 > cell line contains a delT mutation in the core promoter initiator > element. Deletion of T decreases matrix similarity of this DNA element > from 0.945 to 0.941, and, therefore, might negatively influence > expression of KCNRG in RPMI-8226 cells. This suggests that KCNRG > expression may be negatively influenced in this model line. The > haploinsufficiency of KCNRG might be relevant to the progression of > CLL and MM at least in a subset of patients. > > > ### > * > *