April 2011


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Wed, 20 Apr 2011 11:38:05 -0400
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"Diane St. Germain" <[log in to unmask]>
George Mason University
To: Biosciences Graduate Students <[log in to unmask]>, Anna Baranova <[log in to unmask]>, Daniel N Cox <[log in to unmask]>, Alan H Christensen <[log in to unmask]>, Chip Petricoin <[log in to unmask]>, Geraldine M Grant <[log in to unmask]>, Karl J Fryxell <[log in to unmask]>, Monique Van Hoek <[log in to unmask]>, Yuntao Wu <[log in to unmask]>, Charles L Bailey <[log in to unmask]>, Jim Willett <[log in to unmask]>, Serguei Popov <[log in to unmask]>, Lance Liotta <[log in to unmask]>, Valery Soyfer <[log in to unmask]>, Kylene Kehn-Hall <[log in to unmask]>, Fatah Kashanchi <[log in to unmask]>, Alessandra Luchini <[log in to unmask]>, Ramin M Hakami <[log in to unmask]>, Bioinformatics students <[log in to unmask]>, [log in to unmask], Richard Diecchio <[log in to unmask]>, Timothy Born <[log in to unmask]>, Terry R Creque <[log in to unmask]>, Barney M Bishop <[log in to unmask]>, Myung Chul Chung <[log in to unmask]> cc: "Gail L. Hodges" <[log in to unmask]>, Sarah Kostka <[log in to unmask]>, Tiffany C Sandstrum <[log in to unmask]>
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> *Dissertation Defense Announcement
> To:  The George Mason University Community*
> *Candidate: Jessica Helene Chertow
> Program:    PhD Biosciences
> *
> *Date:   Monday May 9, 2011
> Time:   10:00 a.m. 
> Place:  George Mason University
> ** 	     Discovery Hall Auditorium
> 	     Prince William campus <>
> Dissertation Chair: Dr. Serguei Popov
> Dissertation Director: Dr. Myung Chung
> Committee members: Dr. Charles L. Bailey, Dr. Terry Creque, Dr. Barney Bishop*
> *Title: **"/Bacillus Anthracis/ Protease Regulates Bacterial Adhesion and Invasion"* 
> ****
> The dissertation is on reserve in the Johnson Center Library, Fairfax campus.
> The doctoral project will not be read at the meeting, but should be read in advance. 
> /**/
> *All members of the George Mason University community are invited to attend.*
> *
> To initiate an efficient bacterial infection, microbes require the binding and in some cases invasion into host cells. To date, BslA, an S-layer prtoein has been identified as a Bacillus anthracis adhesin.  Here, we investigated the role of immune inhibitor A metalloprotease (InhA) of B. anthracis in adhesion and invasion in endothelial cells. We determined that InhA can serve as a negative regulator of adhesion and invasion in human brain endothelial cells (hBMECs) using wildtype Sterne and inhA-deficient (&#916;inhA) bacilli. The complemention of inhA into mutants induced a similar level of binding as wildtype. BslA levels in S-layer extracts of &#916;inhA strain were significantly higher than wildtype and complemented strains. An inverse correlation between InhA and BslA expression was observed when bacilli were cultured in air or bicarbonate/CO2 conditions. Furthermore, BslA was sensitive to purified InhA degradation in a concentration- and time-dependent manner and transcriptional changes in the BslA gene was not observed. Invaded wildtype bacilli were resistant to intracellular killing compared to &#916;inhA strain. Finally, we found InhA serves an important factor to anthrax virulence in late stage disease in mice.  Taken together, these results suggest InhA regulates B. anthracis adhesion by modifying cell surface properties through direct proteolysis of adhesin protein, BslA and bacteria expressing an hyperadhesive phenotype exhibit decreased virulence in mice.
> ###